Affinity Binder Knock-Down Initiative
نویسندگان
چکیده
منابع مشابه
Two-player Knock ’em Down
We analyze the two-player game of Knock ’em Down, asymptotically as the number of tokens to be knocked down becomes large. Optimal play requires mixed strategies with deviations of order √ n from the naive law-of-large numbers allocation.
متن کاملRapid conditional knock-down–knock-in system for mammalian cells
RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant fo...
متن کاملIdentification of a high-affinity binder for estradiol and a low-affinity binder for testosterone in Coccidioides immitis.
Coccidioides immitis cytosol contains a specific estrogen binder with kinetic characteristics (Kd = 2.1 X 10(-8) to 3.7 X 10(-8) M; n = 0.5 to 1.56 pmol/mg of protein) appropriate for interaction with levels of estrogen encountered during pregnancy. The Kd of a separate androgen binder (ca. 10(-7) M) is probably too low to allow interaction with physiological levels of testosterone.
متن کاملA low-affinity binder for methotrexate in human leukaemia cells.
T h e commonly used trypsin substrate Na-benzoyl-m.arginine p-naphthylamide ( B A N A ) was not cleaved by either GB or SCTPA. BANA caused 50% inhibition of G B at 2 x 1 0 F J M when assayed with MUGB, and 50% inhibition of SCTPA at lo-’ M when assayed with substrate S-2288. We conclude that the proteinases SCTPA and GB (although not necessarily identical) are more closely related t o each othe...
متن کاملShRNA-mediated knock-down of CD200 using the self-assembled nanoparticle-forming derivative of polyethylenimine
Objective(s): ShRNA-mediated silencing strategy is considered to be a potent therapeutic approach. The present study aimed to assess the ability of the previously prepared polyethylenimine (PEI) derivative for the shRNA knock-down of the CD200 gene on the cells obtained from the patients with chronic lymphocytic leukemia (CLL). Materials and Methods: Since there are several investigations...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: EuPA Open Proteomics
سال: 2015
ISSN: 2212-9685
DOI: 10.1016/j.euprot.2015.07.004